4.3 Randomised Block Design

In this design the experimental material is divided into groups or Blocks in such a manner that the experimented units within a particular Block are relatively homogeneous. Each Block contains a complete set of treatment. i.e. it constitute a replication of treatments and the treatments are allocated at random to the experimental units within each Block.

Example 4.6. The varieties of maize results from RBD are:

\(R_1\) \(A_5\) \(C_4\) \(B_5\) \(D_4\) \(E_2\)
\(R_2\) \(B_4\) \(D_3\) \(E_4\) \(C_5\) \(A_6\)
\(R_3\) \(E_3\) \(B_4\) \(C_4\) \(A_3\) \(D_4\)

Test the hypothesis

1.
Between Blocks
2.
Between varieties

Solution. The first thing to get right is which totals matter. The blocks are the rows \(R_1, R_2, R_3\); the treatments are the varieties \(A\) to \(E\). Because the varieties are randomised within each block, they appear in a different order in each row – so the total of a column position is not the total of a treatment, and means nothing at all. The treatment totals have to be gathered by label.

Readings
Block Total
\(R_1\) \(A\) 5 \(C\) 4 \(B\) 5 \(D\) 4 \(E\) 2 20
\(R_2\) \(B\) 4 \(D\) 3 \(E\) 4 \(C\) 5 \(A\) 6 22
\(R_3\) \(E\) 3 \(B\) 4 \(C\) 4 \(A\) 3 \(D\) 4 18

Collected by variety:

Variety \(A\) \(B\) \(C\) \(D\) \(E\) Total
Total \(\tau _j\) 14 13 13 11 9 60

There are \(r=3\) blocks and \(c=5\) treatments, so \(N=15\) and \(T=60\). \[CF=\frac {T^2}{N}=\frac {3600}{15}=240,\] \[SS_{\text {Total}}=\sum \sum x_{ij}^2-CF=254-240=14,\] \[SS_{\text {Blocks}}=\frac {1}{c}\sum _i B_i^2-CF=\frac {20^2+22^2+18^2}{5}-240 =\frac {1208}{5}-240=1.60,\] \[SS_{\text {Treatments}}=\frac {1}{r}\sum _j \tau _j^2-CF =\frac {14^2+13^2+13^2+11^2+9^2}{3}-240=\frac {736}{3}-240=5.33,\] \[SS_{\text {Error}}=14-1.60-5.33=7.07.\] Each total is divided by the number of observations that went into it: block totals by \(c=5\), treatment totals by \(r=3\).

Variation due to df \(SS\) \(MS\) \(F\)
Blocks \(r-1=2\) \(1.60\) \(0.80\) \(0.91\)
Treatments \(c-1=4\) \(5.33\) \(1.33\) \(1.51\)
Error \((r-1)(c-1)=8\) \(7.07\) \(0.88\)
Total \(N-1=14\) \(14.00\)

The degrees of freedom add: \(2+4+8=14\). This is the check that catches the commonest mistake in a two-way table, and it is worth making every time.

At \(\alpha =0.01\):

  • Treatments. \(F=1.51\) against \(F_{4,8,\,0.01}=7.01\). We fail to reject: there is no evidence of a difference between the varieties (\(P=0.29\)).
  • Blocks. \(F=0.91\) against \(F_{2,8,\,0.01}=8.65\). We fail to reject: there is no evidence of a difference between blocks (\(P=0.44\)).

Remark 4.7. That the blocks show no difference is worth a thought rather than a shrug. Blocking is worth doing when the blocks genuinely differ – grouping similar plots together removes that variation from the error term and makes the treatment comparison sharper. Here they barely differ, so the blocking bought little, and the degrees of freedom spent on it (\(2\), taken from the error term) were largely wasted.

That is not a reason to avoid blocking. It has to be decided before the experiment, when whether the plots differ is exactly what is not yet known, and the cost of blocking unnecessarily is small against the cost of failing to block when it mattered.

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